报告题目：三维基因组在生殖干细胞发育过程中的变化规律及其分子机制

报告摘要：

高通量染色质结构捕获技术是以细胞核为研究对象，研究全基因组范围内整个染色质在空间位置上的关系，获得高分辨率的染色质调控元件相互作用图谱，全面阐述染色质三维结构。生殖细胞发育中的染色质三维结构重组变化规律的研究有助于深入理解其发育过程中基因的转录调控机制。本次沙龙报告人主要介绍通过Hi-C, ChIP-seq和ATAC-seq等技术，揭示FGSC特有的染色质高级结构及关键调控因子，并描绘FGSC发育过程中的染色质高级结构变化规律。

报告人简介：

田耿（GENG TIAN）2020年博士毕业于太阳集团城网站2003。长期聚焦动物生殖发育的表观遗传学机制研究，特别是在动物生殖干细胞发育与调控机制和表观遗传如染色质高级结构对配子发生的调控作用。已发表系列SCI论文30篇, 其中Q1论文18篇，包括以(共)第一/通讯作者在Clinical and translational medicine (IF 10.6), Journal of Advanced Research (IF:10.7), Cellular and Molecular Life Science (中科院一区)，Cell Proliferation (中科院一区) 等学术期刊发表论文。

【所在学科研究组研究方向】：

课题组研究方向主要涉及：

1. 动物发育的表观遗传调控机制；

2. 应用三维基因组和单细胞多组学等技术及数据分析探究动物发育科学问题； 

3. 病原微生物与宿主的表观遗传调控机制。

【代表性著作】

1. Tian GG#, Hou C#, Li J*, Wu J*. Three‐dimensional genome structure shapes the recombination landscape of chromatin features during female germline stem cell development. Clinical and translational medicine. 2022; 12: e927. SCI; 影响因子: 10.6 (中科院1区)

2. Tian GG#, Zhao X#, Hou C#, Xie W, Li X, Wang Y, Wang L, Li H, Zhao X*, Li J*, Wu J*. Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells. Cellular and Molecular Life Sciences. 2022; 79: 22. SCI; 影响因子: 9.2 (中科院1区)

3. Luo H#, Li X#, Tian GG#, Li D, Hou C, Ding X, Hou L, Lyu Q, Yang Y, Cooney AJ, Xie W, Xiong J, Wang H, Zhao X*, Wu J*. Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization. Journal of Advanced Research. 2021; 33: 81-98. SCI; 影响因子: 12.8 (中科院1区)  

4. Zhang Y#, Tian GG#, Wang X, Hou C, Hu X*, Wu J*. Retinoic acid induced meiosis initiation of female germline stem cells by remodeling three-dimensional chromatin structure. Cell Proliferation. 2022; 55: e13242. SCI; 影响因子: 8.7 (中科院1区)

5. Tian GG, Li J*, Wu J*. Comparison analysis of multiple-omics data between femaleline stem cells and spermatogonial stem cells. Genes & Diseases. 2022; 10: 727-730. SCI; 影响因子: 7.3

ACADEMIC SALON (LV)

SPEAKER: GENG TIAN

Tenure-track Associate Professor, Ph.D.

Department of Animal Science, SAB

TIME：12:40-14:00 June 26, 2024 (Wed)

VENUE：Room 104, Building B, SAB

ORGANIZER：Office of Discipline and Science & Technology, SAB

Young Teachers Association, SAB

TITLE：3D Genome Structure Shapes the Recombination Landscape of Chromatin Features in Germline Stem Cell Development

ABSTRACT：

High-throughput chromatin conformation capture (Hi-C) technology focuses on the nucleus, investigating the spatial relationships of chromatin throughout the entire genome, and obtaining high-resolution maps of chromatin regulatory elements' interactions, comprehensively elucidating chromatin's three-dimensional structure. Studying the recombination patterns of chromatin's three-dimensional structure during germline development aids in a deeper understanding of the transcriptional regulatory mechanisms of genes during its developmental process. The speaker mainly introduces the chromatin's higher-order structure unique to the female germline stem cells (FGSCs) and key regulatory factors through techniques such as Hi-C, ChIP-seq, and ATAC-seq, and outlines the patterns of chromatin's higher-order structural changes during FGSC development.